Core Flow Cytometry

Infrastructure, services and expertise

Equipment for basic cell analysis

• BD LSR II, 3 laser, (Blue/Red/Violet), MRB II floor 0*
• BD LSR II, 4 laser (Blue/Red/Violet/Yellow-Green), MRB II floor 1*
• BD LSR Fortessa 3 laser (Blue/Red/Violet), MRB II floor 2*
• Myltenyi, MACSQuant 10 (Blue/Red/Violet), MRB II floor 0
• Beckmann Cytoflex (Blue/Red/Violet), Faculty of Veterinary Medicine

*Experiments with 8 or fewer colours perform on the BD Fortessa (3 laser) or on the BD LSR II instruments.

Equipment for advanced cell analysis

• BD LSR Fortessa X-20 5 laser (Blue/Red/Violet/Yellow/Green/UV), MRB II floor 0**
• BD FACSymphony A3 4 laser (Blue, Red, Violet, Yellow-Green), MRB II floor 1**

**Experiments with more than 8 colours are best performed on the BD Fortessa X-20 or the BD FACSymphony A3.

Cell sorting equipment

• BD Facs ARIA III, 4 laser (Blue(488nm)/Red(640nm)/Yellow-Green(561nm)/Violet(405nm)), MRB II floor 1
  
  • Available nozzles : 70, 85 and 100µm
  • 4 sorting channels in tube or microtiter plate
  • Temperature control of sorted cells : from 4°C to 37°C
  • Index sorting (complete phenotype profile of each cell available, single-cell sorting)
• BD Facs FUSION, 4 lasers (Blue/Red/Yellow/Green/Violet), MRB II floor 1
  
  • Available nozzles : 70, 85 and 100µm
  • 4 sorting channels in tube or microtiter plate
  • Temperature control of sorted cells : from 4°C to 37°C
  • Index sorting (complete phenotype profile of each cell available, single-cell sorting)
  • Biosafety cabinet

Terms of use

1. Follow a (basic) training course.

• Basic training 'How to set up a basic analytical Flow Cytometry experiment' to learn the basics of flow cytometry and perform a basic experiment.
• Basic training 'How to prepare a sample for cell sorting.' to learn how to prepare a sample for cell sorting and what to pay attention to in order to obtain a properly sorted sample, such as quality and purity.
• 'Core Flow Cytometry Advanced' training to learn more advanced skills in Flow cytometry. Such training can also be obtained through intervention of the device manufacturer/supplier.
• All training courses are part of the Faculty Research Training Programme, module: Core Facilities. Based on enrollments, several sessions per year will be organised in MRB II - Core Flow Cytometry. Register here.

2. Reserve your use at least 7 working days in advance via the Core Facility Management Software platform (CFMS platform).

• Make your reservation for an instrument, service or product via the CFMS platform. See how to make your reservation here.
• Although the Core Facility staff will perform the cell sorting for you, your presence during the experiment is required to ensure it runs according to your wishes and expectations.

3. Pay a usage fee.

• Use of the Core Facility is possible for a fee. Provide a cost centre for the usage fee within your project application. Check the rates here.

4. Follow the usage procedure for your equipment and the Core Facilities' agreement framework.

• To correctly start up, use and shut down your equipment, follow a defined procedure. View the usage procedure here.
• Specific agreements apply to the use of the Core Facilities. View the agreement framework here.

5. Mention the Core Facility in your scientific output.

• To ensure the funding and continuation of the Core Facility, it is essential to mention this facility as a source. View the guidelines for acknowledgement of Core Facilities in scientific output here.

Contact

• For user info: Core Flow Cytometry user group
• For administration and organisation: Maarten Verdonckt