Department of Human Structure and Repair

Multi-parameter assessment physiological function in spheroids, bioprinted microtissues and in vivo

About this line of research

Bioprinting and the use of numerous biomaterials require the development of online and continuous monitoring tools, reporting on cell health, death, and differentiation and thus predicting the overall quality, reproducibility and long-term stability of bio fabricated tissues. Currently, we focus on optimising multi-parameter O2 and T (temperature) imaging in bioprinted constructs, organoids and differentiating homo- and heterocellular spheroids.

We employ multiplexing in spectral and luminescence lifetime domains and aim at expanding the biosensor probes toolkit, e.g. by utilising staining of the cells, use of genetically encoded proteins, functional autofluorescence, labelling of extracellular matrix components and introduction of advanced nanoparticle sensors.

Here we also collaborate with research groups who design and model microfluidic chips, and microscopes as well as develop innovative biomaterials and biosensors, including materials for two- and three-photon excitation modes (FLIMagin3D consortium).

Publications

  1. Debruyne AC, Okkelman IA, Dmitriev RI: Balance between the cell viability and death in 3D. Seminars in Cell & Developmental Biology
    1. Dmitriev RI, Intes X, Barroso MM: Luminescence lifetime imaging of three-dimensional biological objects. Journal of Cell Science 2021, 134(9):1-17.
    2. Perottoni S, Neto NGB, Di Nitto C, Dmitriev RI, Raimondi MT, Monaghan MG: Intracellular label-free detection of mesenchymal stem cell metabolism within a perivascular niche-on-a-chip. Lab on a Chip 2021, 21(7):1395-1408.
    3. Müller BJ, Zhdanov AV, Borisov SM, Foley T, Okkelman IA, Tsytsarev V, Tang Q, Erzurumlu RS, Chen Y, Zhang H et al: Nanoparticle-Based Fluoroionophore for Analysis of Potassium Ion Dynamics in 3D Tissue Models and In Vivo. Advanced Functional Materials 2018, 28(9):1704598.
    4. Jenkins J, Borisov SM, Papkovsky DB, Dmitriev RI: Sulforhodamine nanothermometer for multi-parametric Fluorescence Lifetime Imaging Microscopy. Analytical Chemistry 2016, 88(21):10566-10572.
    5. Dmitriev RI, Zhdanov AV, Nolan YM, Papkovsky DB: Imaging of neurosphere oxygenation with phosphorescent probes. Biomaterials 2013, 34(37):9307-9317.

    Questions?

    • Ruslan Dmitriev, professor/head of the group

    +32 9 332 51 33